Get tips on using HyClone™ Dulbecco's Modified Eagles Medium to perform Mammalian cell culture media MDA-MB-231
I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?
Get tips on using MicroSEQ™ D2 rDNA Fungal PCR Kit to perform Cell Culture Contamination Detection Kit Fungi
Get tips on using Fungi-Fluor® Kit for Fungal Detection to perform Cell Culture Contamination Detection Kit Fungi
Get tips on using VetMAX™-Gold BVDV PI Detection Kit to perform Cell Culture Contamination Detection Kit Virus
Get tips on using MycoAlert® Mycoplasma Detection Kit (50 Tests) to perform Cell Culture Contamination Detection Kit Mycoplasma
Get tips on using MycoAlert® Mycoplasma Detection Kit (50 Tests) to perform Cell Culture Contamination Detection Kit Mycoplasma
Get tips on using Macrolide – R/MG ELITe MGB®Kit to perform Cell Culture Contamination Detection Kit Mycoplasma
Get tips on using Difco™ Brain Heart Infusion Agar, BD to perform Bacterial cell culture media Escherichia coli
Get tips on using Difco™ Brain Heart Infusion Agar, BD to perform Bacterial cell culture media Streptococcus pyogenes
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