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Get tips on using Quick-DNA™ Fungal/Bacterial Miniprep Kit to perform DNA isolation / purification Yeast - Candida albicans

Get tips on using FastDNA™ SPIN Kit for Feces to perform DNA isolation / purification Bacteria - Gram positive Clostridium difficile

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized HeLa, CaSki and C33A (Cervical cancer cells)

Get tips on using QIAamp DNA Mini Kit to perform DNA isolation / purification Cells - Immortalized cell lines Renal cortical tubule epithelial cells

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Bacteria - Gram positive piezophilic bacteria [AT7 and AT12 Strains]

Get tips on using Direct-zol™ RNA MiniPrep Plus to perform RNA isolation / purification Bacteria - Gram negative Escherichia coli

Get tips on using MICROBExpress™ Bacterial mRNA Enrichment Kit to perform RNA isolation / purification Bacteria - Gram positive Staphylococcus aureus

Get tips on using RiboMinus™ Transcriptome Isolation Kit, bacteria to perform RNA isolation / purification Bacteria - Gram negative Pseudomonas aeruginosa

Get tips on using MICROBExpress™ Bacterial mRNA Enrichment Kit to perform RNA isolation / purification Bacteria - Gram negative Pseudomonas aeruginosa

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.