DNA isolation / purification Plants

Get tips on using s4215 to perform siRNA / miRNA gene silencing Human - PANC-1 DNMT1/3b

Get tips on using Hs_DNMT3B_2 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - HCT-116 DNMT3B

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

Get tips on using CD38 CRISPR Activation Plasmid (r) to perform CRISPR Rat - Activation CD38

Get tips on using IFITM1 CRISPR Activation Plasmid (h) to perform CRISPR Human - Activation IFITM1

Get tips on using CMV-CAS9-2A-GFP Plasmid to perform CRISPR Human - Deletion TRIB1

Get tips on using AChE shRNA Plasmids (h) to perform shRNA gene silencing Human - TF‐1 AChE

Get tips on using PlasmoTest™ - Mycoplasma Detection Kit to perform Cell Culture Contamination Detection Kit Mycoplasma

Get tips on using HE4 CRISPR/Cas9 KO Plasmid (h) to perform CRISPR Human - Repression HE4

Get tips on using dCK CRISPR/Cas9 KO Plasmid (h) to perform CRISPR Human - Repression DCK