rna-isolation-purification-cells-primary-mouse-cortical-neurons

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Get tips on using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells to perform Live / Dead assay mammalian cells - THP-1

Products Thermo Fisher Scientific LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells

Get tips on using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells to perform Live / Dead assay mammalian cells - fibroblast Balb/3T3

Products Thermo Fisher Scientific LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells

Get tips on using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells to perform Live / Dead assay mammalian cells - human fibroblast tissue

Products Thermo Fisher Scientific LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells

Get tips on using Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells to perform Live / Dead assay mammalian cells - human fibroblast tissue

Products Biotium Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Cellular assays Autophagy assay cell type U2OS (human bone osteosarcoma epithelial cells)

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-lactamase substrates HEK 293 & HEK 293T cells

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-galactosidase substrates HEK293 human embryonic kidney cells

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-galactosidase substrates U87 and U251 glioblastoma cells

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-galactosidase substrates human MSCs (mesenchymal stem cells)

Get tips on using LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells to perform Live / Dead assay mammalian cells - K562

Products Thermo Fisher Scientific LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells

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