DNA transfection Mammalian cells Immortalized cell lines

Get tips on using CometAssay Electrophoresis System II to perform DNA Damage Assay SJSA-1

Get tips on using CometAssay Electrophoresis System II to perform DNA Damage Assay Saos-2

Get tips on using CometAssay Electrophoresis System II to perform DNA Damage Assay HEK 293T

Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay COV362

Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay COV362

Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay A549

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using Hoechst 33258 to perform DNA quantification Human - MDA-MB-231

Get tips on using Sodium bisulfite to perform PCR Methylation specific PCR - Bacterial DNA

Get tips on using HaeIII Methyltransferase to perform PCR Methylation specific PCR - Bacterial DNA