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As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Cellular assays Autophagy assay cell type MT-2 (human T cell leukaemia)

Get tips on using Mitochondrial ROS Activity Assay Kit (Deep Red Fluorescence) to perform ROS assay cell type - mouse cardiomyocytes

Products eEnzyme Mitochondrial ROS Activity Assay Kit (Deep Red Fluorescence)

Get tips on using CultreCoat Medium BME Cell Invasion Assay, 96 well to perform Cell migration / Invasion cell type - HCT116

Products R&D Systems CultreCoat Medium BME Cell Invasion Assay, 96 well

Get tips on using CultreCoat Low BME Cell Invasion Assay, 96 well to perform Cell migration / Invasion cell type - HN4

Products R&D Systems CultreCoat Low BME Cell Invasion Assay, 96 well

Get tips on using CultreCoat Low BME Cell Invasion Assay, 96 well to perform Cell migration / Invasion cell type - SCC2

Products R&D Systems CultreCoat Low BME Cell Invasion Assay, 96 well

Get tips on using CultreCoat Low BME Cell Invasion Assay, 96 well to perform Cell migration / Invasion cell type - SCC104

Products R&D Systems CultreCoat Low BME Cell Invasion Assay, 96 well

Get tips on using CultreCoat Low BME Cell Invasion Assay, 96 well to perform Cell migration / Invasion cell type - SCC6

Products R&D Systems CultreCoat Low BME Cell Invasion Assay, 96 well

Get tips on using CultreCoat Low BME Cell Invasion Assay, 96 well to perform Cell migration / Invasion cell type - SCC4

Products R&D Systems CultreCoat Low BME Cell Invasion Assay, 96 well

Get tips on using CultreCoat BME Cell Invasion Optimization Assay, 96 well to perform Cell migration / Invasion cell type - H1299

Products R&D Systems CultreCoat BME Cell Invasion Optimization Assay, 96 well

Get tips on using CultreCoat Medium BME Cell Invasion Assay, 96 well to perform Cell migration / Invasion cell type - Skov3

Products R&D Systems CultreCoat Medium BME Cell Invasion Assay, 96 well

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