ChIP H3K27me3 Human Bovine

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using Anti-Human L1CAM Therapeutic Antibody Fab Fragment to perform Flow cytometry Anti-bodies Human - CD171/L1CAM

Get tips on using Human Melanocyte Media to perform Mammalian cell culture media HEM

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Get tips on using Human PDGF-BB DuoSet ELISA to perform ELISA Human - PDGF-BB

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