RNA sequencing Human

Get tips on using NucleoSpin® RNA XS to perform RNA isolation / purification Tissue - Human Skin

Get tips on using PAXgene Tissue RNA Kit to perform RNA isolation / purification Tissue - Human Colon

Get tips on using NucleoSpin® RNA/Protein to perform RNA isolation / purification Tissue - Human Cornea

Get tips on using GeneChip® Human Genome U133 Plus 2.0 Array to perform RNA amplification & labeling Mammalian - RNA amplification and Labeling Human Endometrial Stromal cells Biotin

Get tips on using GeneChip® Human Genome U133 Plus 2.0 Array to perform RNA amplification & labeling Mammalian - RNA, rhesus monkey brain tissue Human endothelail stromal cells

Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - human blood

Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - human plasma

Get tips on using Agilent RNA 6000 Pico Kit to perform RNA quantification Fuorimetric - human saliva

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.