Protein Expression Prokaryotic cells E. coli

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Hello everyone! I am going to do a live/dead assay for my cells and I saw that I can use both fluorescence and absorbance as my detection method. Is there a difference in the results depending on the method? Is one method preferred over the other in certain situations?

There are a plethora of detection methods of cell cytotoxicity and proliferation by flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using QIAprep Spin Miniprep Kit to perform Plasmid Isolation Proteus mirabilis

Get tips on using GeneJET Plasmid Miniprep Kit to perform Plasmid Isolation Proteus mirabilis

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Rat Colon

Get tips on using RNA-Bee to perform RNA isolation / purification Tissue - Mouse Colon

Get tips on using SIHK1738 to perform siRNA / miRNA gene silencing Human - Colon-26 PKN3

Get tips on using Lentivirus Rapid Quantitation Kit to perform RNA quantification Coloremetric

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Rat Colon