PCR Quantitative real-time PCR

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using AllPrep DNA/RNA Micro Kit to perform RNA isolation / purification Tissue - Human Blood / Serum / Plasma / Buffy coat

Get tips on using DNeasy Blood and Tissue Kit (250) to perform DNA isolation / purification Cells - Immortalized cell lines Loucy

Get tips on using mericon DNA Bacteria Plus Kit (50) to perform DNA isolation / purification Bacteria - Gram positive Clostridium botulinum

Get tips on using EZ1 DNA Blood 200 µl Kit (48) to perform DNA isolation / purification Tissue - blood / plasma

Get tips on using QIAamp DNA Blood BioRobot MDx Kit (12) to perform DNA isolation / purification Tissue - blood / plasma

Get tips on using QIAamp DNA Blood BioRobot 9604 Kit (12) to perform DNA isolation / purification Tissue - blood / plasma

Get tips on using QIAamp 96 DNA QIAcube HT Kit (5) to perform DNA isolation / purification Tissue - blood / plasma

Get tips on using QIAamp 96 PowerFecal QIAcube HT Kit (5) to perform DNA isolation / purification Micorbiome - Human gut

Get tips on using QIAamp Fast DNA Stool Mini Kit (50) to perform DNA isolation / purification Tissue - fecal sample