siRNA / RNAi /miRNA transfection Human Cells KG-1

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human BxPC-3

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human epidermal keratinocytes

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human Caco-2

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human MCF-7

Get tips on using Corning® Hepatocyte Culture Media Kit, 500 mL to perform 3D Cell Culture Media Human prostate organoid

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized C3H-10T1/2

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized MDA-MB-157

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized 293T