Protein expression and purification Mammalian cells

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using TAGZyme Kit to perform Protein tag His-tag removal

Get tips on using Trichloroacetic acid to perform Protein isolation Bacteria - Clostridium difficile

Get tips on using Trichloroacetic acid to perform Protein isolation Bacteria - Chlamydia pneumoniae

Get tips on using Trichloroacetic acid to perform Protein isolation Bacteria - Bacillus subtilis

Get tips on using Qproteome Mitochondria Isolation Kit to perform Protein enrichment Mitochondria

Get tips on using Ni-NTA HisSorb Strips (24) to perform Protein tag Detection of His-tagged proteins