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Site Directed Mutagenesis (SDM) Rat

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DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA Microarray Comperative genomic hybridization Human BT474

Cell culture media 3D Cell Culture Media Human small intestinal organoids

Cell culture media 3D Cell Culture Media Mouse small intestinal organoids

Get tips on using Smooth Muscle Cell Medium to perform Mammalian cell culture media HAOSMC

Products ScienCell Research Laboratories Smooth Muscle Cell Medium

Get tips on using SMARTer® PCR cDNA Synthesis Kit to perform cDNA synthesis Yeast

Products Takara Bio Inc SMARTer® PCR cDNA Synthesis Kit

Get tips on using TruSeq Small RNA Library Preparation Kit to perform RNA sequencing Human - HEK293T

Products Illumina TruSeq Small RNA Library Preparation Kit

Get tips on using Smooth Muscle Cell Growth Medium 2 to perform Mammalian cell culture media HCASMC

Products PromoCell Smooth Muscle Cell Growth Medium 2

Get tips on using Canine Smooth Muscle Cell Growth Medium to perform Mammalian cell culture media CnAOSMC

Products Cell Applications Inc Canine Smooth Muscle Cell Growth Medium

Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA Microarray RNA amplification & Labeling Fish fundulus heteroclitus Cyanine-3 / Cyanine-5

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA Microarray Gene expression arrays Mouse liver tissue Cyanine-3-CTP

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