siRNA / miRNA gene silencing Rat Brain endothelial cells HIF-1α

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

Get tips on using Live/Dead Cell Double Staining Kit to perform Live / Dead assay mammalian cells - L29 mouse fibroblast

Get tips on using Live-Dead cell staining kit (Enzo) to perform Live / Dead assay mammalian cells - human fibroblast tissue

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - L29 mouse fibroblast

Get tips on using QuickGene RNA Cultured Cell HC Kit S to perform RNA isolation / purification Cells - immortalized SH-SY5Y

Get tips on using RealTime-Glo™ MT Cell Viability Assay to perform Live / Dead assay mammalian cells - HEK 293

Get tips on using RealTime-Glo™ MT Cell Viability Assay to perform Live / Dead assay mammalian cells - THP-1

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Mouse primary breast cancer ephitelial cells-Mammospheres

Get tips on using Anti-LC3B antibody (ab48394) to perform Autophagy assay cell type - U2OS (human bone osteosarcoma epithelial cells)

Get tips on using Lipofectamine® LTX Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines MDA-MB-231