Protein expression and purification Mammalian cells HEK 293

Get tips on using Tissue Extraction Reagent I to perform Protein isolation Tissue - Mouse liver tissue

Get tips on using TAGZyme DAPase Enzyme (50 U) to perform Protein tag His-tag removal

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Human umbilical cord tissue

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Rabbit eye retina/choroids

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Mouse Cerebral hemispheres

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Cerebral hemispheres

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Heart

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Heart

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Mouse Heart