shRNA gene silencing Mouse Prostate cancer cell lines (DU145 and PC3)

Get tips on using Smooth Muscle Actin Antibody (B4): sc-53142 to perform Immunohistochemistry Mouse - SMA

Get tips on using Polyclonal Rabbit Anti-Human Myeloperoxidase (Dako Omnis) to perform Immunohistochemistry Mouse - MPO

Get tips on using pcDNA™3.1 (+) Mammalian Expression Vector to perform Protein expression and purification Mammalian cells - CHO-K1 sRAGE

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - primary mouse cortical neurons

Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Cells - primary mouse cardiac fibroblasts

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - primary mouse oocytes

Get tips on using TaqMan® MicroRNA Reverse Transcription Kit to perform siRNA / RNAi /miRNA transfection Mouse - Glomerular Mesangial cells polymer / lipid

Get tips on using PCNA Antibody (F-2) to perform Cell cytotoxicity / Proliferation assay cell type - oral squamous cell carcinoma

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.

Get tips on using Goat Anti-Type III Collagen to perform Immunohistochemistry Collagen Type I - Goat Mouse -NA-