Site Directed Mutagenesis (SDM) Human Point mutation BxPC-3

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

Get tips on using hCas9 to perform CRISPR Mouse - Deletion 3T3-L1 ATP7A

Get tips on using Comet Assay to perform DNA Damage Assay A-375

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - 3T3-L1

Get tips on using lentiCRISPR v2 to perform CRISPR Mouse - Deletion 3T3-L1 TEAD

Get tips on using Advanced DMEM to perform 3D Cell Culture Media Murina esophageal organoids

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized NIH-3T3

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized C6/36

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Mouse - 3T3-L1

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Mouse - NSC-34