CRISPR Mouse Deletion

Get tips on using Mouse Lipocalin-2 ELISA Kit (NGAL) (ab199083) to perform ELISA Mouse - NGAL/LCN2

Get tips on using Mouse KIM1 ELISA Kit (TIM-1) (ab119596) to perform ELISA Mouse - KIM-1

Get tips on using Mouse IGF-1 PicoKine™ ELISA Kit to perform ELISA Mouse - IGF-I

Get tips on using Mouse Heme Oxygenase 1 ELISA Kit (ab204524) to perform ELISA Mouse - HO-1

Get tips on using Rat/Mouse Cytochrome c Quantikine ELISA Kit to perform ELISA Mouse - Cytochrome c

Get tips on using Mouse BMP-2 PicoKine™ ELISA Kit to perform ELISA Mouse - BMP-2

Get tips on using Mouse TGF beta 1 ELISA Kit (ab119557) to perform ELISA Mouse - TGF-beta 1

Get tips on using Mouse IL-1 beta ELISA Kit (ab100704) to perform ELISA Mouse - IL-1 beta

Get tips on using Mouse ICAM-1/CD54 Quantikine ELISA Kit to perform ELISA Mouse - ICAM-1/CD54

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).