Protein isolation Yeast

Get tips on using B-PER™ II Bacterial Protein Extraction Reagent (2X) to perform Protein isolation Bacteria - Synechococcus elongatus

Get tips on using B-PER™ II Bacterial Protein Extraction Reagent (2X) to perform Protein isolation Bacteria - Salmonella enterica

Get tips on using B-PER™ II Bacterial Protein Extraction Reagent (2X) to perform Protein isolation Bacteria - Pseudomonas aeruginosa

Get tips on using B-PER™ II Bacterial Protein Extraction Reagent (2X) to perform Protein isolation Bacteria - Escherichia coli

Get tips on using M-PER™ Mammalian Protein Extraction Reagent to perform Protein isolation Mammalian cells - Human aortic endothelial cells

Get tips on using Gateway™ pDONR™221 Vector to perform Protein expression and purification Yeast - Saccharomyces cerevisiae ΔHSPA5

Get tips on using pPICZα A, B, & C Pichia Vectors to perform Protein expression and purification Yeast - Pichia pastoris hmPRα

Get tips on using PRO-PREP™ Protein Extraction Solution (C/T) to perform Protein isolation Mammalian cells - Mouse Epididymal fat

Protein ladders are a set of standards known as molecular weight proteins that are utilized to identify the approximate size of a protein molecule run on a PAGE gel electrophoresis. The challenges in running the ladders are the choice of appropriate protein standard as it is used as visual evidence of protein migration, transfer efficiency, and positive control. Suitable protein markers can be selected on the basis of required properties and applications, i.e., fluorescent ladder, IEF, 2D SDS-PAGE ladder, natural ladder with an isoelectric point, and optimized ladders for Western Blot chemiluminescence detection. The key factors for running a distinct protein ladder are buffer conditions, charge/voltage at migration time, and the gel's concentration.

Protein ladders are a set of standards known as molecular weight proteins that are utilized to identify the approximate size of a protein molecule run on a PAGE gel electrophoresis. The challenges in running the ladders are the choice of appropriate protein standard as it is used as visual evidence of protein migration, transfer efficiency, and positive control. Suitable protein markers can be selected on the basis of required properties and applications, i.e., fluorescent ladder, IEF, 2D SDS-PAGE ladder, natural ladder with an isoelectric point, and optimized ladders for Western Blot chemiluminescence detection. The key factors for running a distinct protein ladder are buffer conditions, charge/voltage at migration time, and the gel's concentration.