Get tips on using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) to perform Cell cytotoxicity / Proliferation assay cell type - Hep G2
Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Rat Heart
Get tips on using Tissue Extraction Reagent I to perform Protein isolation Tissue - Mouse liver tissue
Get tips on using TAGZyme DAPase Enzyme (50 U) to perform Protein tag His-tag removal
A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Mouse Cerebral hemispheres
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Cerebral hemispheres
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Heart
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Heart
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment