siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells GLO-1

Get tips on using FITC Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - OECM-1

Get tips on using FITC Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - THP-1

Get tips on using ApoAlert™ Annexin V-FITC Apoptosis Kit to perform Apoptosis assay cell type - PANC-1

Get tips on using Mouse PAI1 ELISA Kit (SERPINE1) (ab197752) to perform ELISA Mouse - Serpin E1/PAI-1

Get tips on using Rat PAI1 ELISA Kit (SERPINE1) (ab198509) to perform ELISA Rat - Serpin E1/PAI-1

Get tips on using EZ-Magna ChIP™ A - Chromatin Immunoprecipitation Kit to perform ChIP Mouse - Hepa-1

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized OS-RC-2

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Rat - Deletion INS-1 832/13 Ep300

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Rat - INS-1 832/13