Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized EBL (embryonic lung cell)
A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using Cell Lysis Buffer (10X) to perform Protein isolation Mammalian cells - HepG2
Get tips on using Mammalian Cell Lysis kit to perform Protein isolation Mammalian cells - STTG1
Get tips on using SQSTM1/p62 Antibody to perform Autophagy assay cell type - K562 cells
A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) to perform ROS assay cell type - PLHC-1, SK-HEP-1, Hep3b, HepG2 human hepatocellular carcinoma
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary bovine monocyte derived macrophages
Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized C-33 A
Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized SK-BR-3
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