siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells GLO-1

Get tips on using In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based to perform Cell cytotoxicity / Proliferation assay cell type - THP-1

Get tips on using pSS2-GLURP- SERA5 to perform Protein Expression Prokaryotic cells - L. lactis SERA5 P. falciparum

Get tips on using pSS2-GLURP- MSPDBL2 to perform Protein Expression Prokaryotic cells - L. lactis MSPDBL2 P. falciparum

Get tips on using pET32a (+)-Glu-Em7 to perform Protein Expression Prokaryotic cells - E. coli B. subtilis Em7

Get tips on using GeneBLAzer In Vivo Detection Kit to perform Reporter gene assay β-lactamase substrates - HeLa

Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Whole genome profiling - mouse primordial germ cells

Get tips on using Purified anti-mouse Ly-6C Antibody to perform Flow cytometry Anti-bodies Mouse - Ly6C/Gr-1/Ly6G

Get tips on using Purified anti-mouse Ly-6G Antibody to perform Flow cytometry Anti-bodies Mouse - Ly6C/Gr-1/Ly6G

Get tips on using PE Rat Anti-Mouse Ly-6G to perform Flow cytometry Anti-bodies Mouse - Ly6C/Gr-1/Ly6G

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.