Generally it has been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Use of enzymes to disrupt cell wall can alter gene expression profiles. Therefore, physical disruption can result in high quality RNA for all downstream processing. Use of DNAse and proteinase K will remove traces of DNA contamination and proteins respectively.
Get tips on using Aurum™ Total RNA Fatty and Fibrous Tissue Kit to perform RNA isolation / purification Tissue - Human Adipose
Get tips on using Enzo BioArray™ Single-Round RNA Amplification and Biotin Labeling System to perform Microarray RNA amplification & Labeling - Rhesus monkey brain tissue Biotin
Get tips on using Enzo BioArray™ Single-Round RNA Amplification and Biotin Labeling System to perform Microarray Rhesus monkey - Brain tissue Target preparation (RNA amplification + labeling)
Get tips on using Aurum™ Total RNA Fatty and Fibrous Tissue Kit to perform RNA isolation / purification Tissue - Human Uterus
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Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - mouse adipose tissue
Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Bacteria - Gram positive Bacillus anthracis
Get tips on using Cytoplasmic and Nuclear RNA Purification Kit to perform RNA isolation / purification Cells - immortalized Akata
Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - human colorectal adenocarcinoma cells (CL-187)
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