siRNA / miRNA gene silencing Human ES2(ovarian cancer cell line)

Get tips on using B2M siRNA to perform siRNA / miRNA gene silencing Human - hES cell line H1 (WA01) B2M

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.