As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.
ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.
Get tips on using dCas9 plasmid to perform CRISPR Human - Repression BRCA1
Get tips on using lenti dCAS-VP64_Blast to perform CRISPR Human - Activation ASCL1
Get tips on using lenti dCAS-VP64_Blast to perform CRISPR Human - Activation RhoGDIα
Get tips on using pSLQ1658-dCas9-EGFP to perform CRISPR Human - Activation BRCA1
Get tips on using pcDNA-dCas9-VP64 to perform CRISPR Human - Activation REPRIMO
Get tips on using pMCSG7-D1aerobic to perform Protein Expression Prokaryotic cells - E. coli D1
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment