Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - Mouse skeletal muscle cells
Get tips on using Cell Meter™ Autophagy Assay Kit *Green Fluorescence to perform Autophagy assay cell type - Mesenchymal stromal cells
Get tips on using Live/Dead cell Staining Kit II to perform Live / Dead assay mammalian cells - SH-SY5Y Human neuroblastoma
Get tips on using LIVE/DEAD™ Cell Imaging Kit to perform Live / Dead assay mammalian cells - SH-SY5Y Human neuroblastoma
Get tips on using Live/Dead cell Staining Kit II to perform Live / Dead assay mammalian cells - bmMSCs human bone marrow
Get tips on using LIVE/DEAD Fixable Violet Dead Cell Stain Kit to perform Live / Dead assay mammalian cells - HEK 293
Get tips on using LIVE/DEAD Fixable Violet Dead Cell Stain Kit to perform Live / Dead assay mammalian cells - mouse splenocytes
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
Get tips on using PowerPlex® 18D System to perform Cell line authentication Human iPSC cells derived from human dermal fibroblasts
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment