Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - WI-38
Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - HEK 293
Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - THP 1
Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Mouse - NIH 3T3
Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - Hep G2
Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - human blood
Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - human plasma
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment