ELISA (kit) IL-6, IL-1β, IL-8 and TNF-α -NA- Human

Get tips on using 8 µm Chemotaxis Assays, 24-Well Format to perform Cell migration / Invasion cell type - FTC133

Get tips on using 8 µm Chemotaxis Assays, 24-Well Format to perform Cell migration / Invasion cell type - FTC236

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using 8 µm Chemotaxis Assays, 96-Well Format to perform Cell migration / Invasion cell type - MCF-10A

Get tips on using 8 µm Chemotaxis Assays, 24-Well Format to perform Cell migration / Invasion cell type - PANC-1

Get tips on using ALCAM Antibody (B-6): sc-74558 to perform Immunohistochemistry Mouse - CD166 / ALCAM

Get tips on using FuGENE® 6 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat cardiomyocytes

Get tips on using FuGENE® 6 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat articular chondrocytes

Get tips on using CD14 Monoclonal Antibody (Sa2-8), APC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD14

Get tips on using CD14 Monoclonal Antibody (Sa2-8), FITC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD14