DNA isolation / purification Cells Primary cells

Get tips on using QuantiTect Multiplex PCR NoROX Kit (1000) to perform PCR Multiplex PCR - Poultry DNA

Get tips on using SYBR GreenER™ qPCR SuperMix Universal to perform PCR Multiplex PCR - Bacterial DNA

Get tips on using Ion AmpliSeq™ Library Kit 2.0 to perform PCR Multiplex PCR - Mammalian DNA

Get tips on using Phusion U Multiplex PCR Master Mix to perform PCR Multiplex PCR - Mammalian DNA

Get tips on using BH ladder :: GD OneMARK B RTU Ladder to perform DNA Ladder 1 kb

Get tips on using Quant-iT™ PicoGreen® dsDNA Assay Kit to perform DNA quantification Blood

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using pQE-80L vector to perform Protein expression and purification Bacteria - Escherichia coli IFABP-Aβ

Get tips on using pPIC9K Pichia Vector to perform Protein expression and purification Yeast - Pichia pastoris N-APP