Generally it has been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Use of enzymes to disrupt cell wall can alter gene expression profiles. Therefore, physical disruption can result in high quality RNA for all downstream processing. Use of DNAse and proteinase K will remove traces of DNA contamination and proteins respectively.
Generally it has been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Use of enzymes to disrupt cell wall can alter gene expression profiles. Therefore, physical disruption can result in high quality RNA for all downstream processing. Use of DNAse and proteinase K will remove traces of DNA contamination and proteins respectively.
Generally it has been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Use of enzymes to disrupt cell wall can alter gene expression profiles. Therefore, physical disruption can result in high quality RNA for all downstream processing. Use of DNAse and proteinase K will remove traces of DNA contamination and proteins respectively.
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Mouse Muscle
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Mouse Lung
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Mouse Liver
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Mouse Vagina
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Mouse Tongue
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Mouse Tendons
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Mouse Stomach
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