When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
Get tips on using RNeasy Lipid Tissue Mini Kit to perform RNA isolation / purification Cells - primary human vascular endothelial cells
Get tips on using RNeasy Lipid Tissue Mini Kit to perform RNA isolation / purification Cells - primary bovine aortic endothelial cells
Get tips on using Total RNA Purification Kit to perform RNA isolation / purification Tissue - Human Adrenal glands
Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Brain
Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Thymus
Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Spleen
Get tips on using AllPrep DNA/RNA FFPE Kit to perform RNA isolation / purification Tissue - Mouse Prostate
Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Ovarian
Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Lung
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