siRNA / miRNA gene silencing Rat B35

Get tips on using ON-TARGETplus Rat Pld2 (25097) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - RBL-2H3 Pld2

Get tips on using ON-TARGETplus Rat Atg7 (312647) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - UMR‐106 Atg7

Get tips on using ON-TARGETplus Rat Becn1 (114558) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - UMR‐106 Becn1

Get tips on using ON-TARGETplus Rat Arhgef12 (367072) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 Larg/Arhgef12

Get tips on using ON-TARGETplus Rat Arhgef1 (60323) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 p115RhoGEF/Arhgef1

Get tips on using ON-TARGETplus Rat Tmed10 (84599) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - NRK Tmp21/Tmed10

Get tips on using Flot1 Rat siRNA Oligo Duplex (Locus ID 64665) to perform siRNA / miRNA gene silencing Rat - NRCM Flot1

Get tips on using Flot2 Rat siRNA Oligo Duplex (Locus ID 83764) to perform siRNA / miRNA gene silencing Rat - NRCM Flot2

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.