Protein Expression Prokaryotic cells B. licheniformis

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using Tryptic Soy Broth for microbiology to perform Bacterial cell culture media Salmonella Typhi

Get tips on using LB Broth with agar (Lennox) to perform Bacterial cell culture media Vibrio cholera

Get tips on using LB Broth with agar (Lennox) to perform Bacterial cell culture media Salmonella Typhi

Get tips on using Todd-Hewitt Broth (T20-106) to perform Bacterial cell culture media Streptococcus pyogenes

Get tips on using Neural Maintenance-XF Medium to perform 3D Cell Culture Media Human blood-brain barrier organoid

Get tips on using APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU to perform DNA Damage Assay Human Skin Fibroblast Cell (FSK)

Get tips on using APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU to perform TUNEL assay cell type - SK-MEL-2 human melanoma

Get tips on using MRS BROTH (DE MAN, ROGOSA, SHARPE) to perform Bacterial cell culture media Lactobacillus plantarum

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - BxPC-3