siRNA / miRNA gene silencing Human T47-D

Get tips on using EZ1 DSP DNA Blood Kit (48) to perform DNA isolation / purification Tissue - blood / plasma

Get tips on using cyclin D1 Antibody (DCS-6): sc-20044 to perform Western blotting Cyclin D1

Get tips on using Live/Dead Double Staining Kit (Merck) to perform Live / Dead assay bacteria - Fusobacterium nucleatum

Get tips on using Live/Dead Double Staining Kit (Merck) to perform Live / Dead assay bacteria - Pseudomonas aeruginosa

Get tips on using Live/Dead Double Staining Kit (Merck) to perform Live / Dead assay bacteria - Porphyromonas gingivalis

Get tips on using Live/Dead Cell Double Staining Kit to perform Live / Dead assay mammalian cells - HUVEC

Get tips on using Phusion Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Mouse - Deletion C2C12 DMD

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.