Protein Expression Eukaryotic cells K. lactis

Get tips on using FreeStyle™ 293-F Cells to perform Protein expression and purification Mammalian cells - HEK 293 EGFR

Get tips on using Nucleic Acid Purification to perform Plasmid Isolation Lactococcus lactis

Get tips on using QIAprep Spin Miniprep Kit to perform Plasmid Isolation Lactococcus lactis

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using EasySelect™ Pichia Expression Kit to perform Protein expression and purification Yeast - Pichia pastoris Chymase

Get tips on using pET-32c to perform Protein Expression Prokaryotic cells - E. coli rpf-like protein

Get tips on using pCEP4 Mammalian Expression Vector to perform Protein expression and purification Bacteria - Origami™ B (DE3) ABCA4

Get tips on using pFastBac™ Dual Expression Vector to perform Protein expression and purification Bacteria - DH10Bac™ GYS1-GN1

Get tips on using Champion™ pET SUMO Expression System to perform Protein expression and purification Bacteria - Escherichia coli IFNA2

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized K-562