DNA transfection Mammalian cells

Contamination can affect cell characteristics, i.e., growth, metabolism, and morphology leading to unreliable and erroneous experimental data. Depending on the source of contaminants, one can detect contamination by using a light microscope, gram stain, isothermal amplification, or PCR. Bacteria and fungi can usually be identified by optical microscopy. Mycoplasma in cell cultures cannot be detected visually. Hence, these microbes can go unnoticed for long periods and are determined using dedicated assays. Early and rapid identification of contaminants is vital to detect, handle and prevent contamination for good cell-culture practices. However, detection and identification can be challenging and tricky based on usual visual identifications. Hence it is essential to use a standard contamination detection kit to detect and maintain best practices.

Contamination can affect cell characteristics, i.e., growth, metabolism, and morphology leading to unreliable and erroneous experimental data. Depending on the source of contaminants, one can detect contamination by using a light microscope, gram stain, isothermal amplification, or PCR. Bacteria and fungi can usually be identified by optical microscopy. Mycoplasma in cell cultures cannot be detected visually. Hence, these microbes can go unnoticed for long periods and are determined using dedicated assays. Early and rapid identification of contaminants is vital to detect, handle and prevent contamination for good cell-culture practices. However, detection and identification can be challenging and tricky based on usual visual identifications. Hence it is essential to use a standard contamination detection kit to detect and maintain best practices.

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Get tips on using BacTiter-Glo™ Microbial Cell Viability Assay to perform Live / Dead assay bacteria - Staphylococcus epidermidis

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Get tips on using DraI (10 U/µL) to perform Restriction Enzymes DraI

Get tips on using DraI (AhaIII) restriction enzyme to perform Restriction Enzymes DraI

Get tips on using Bacteria Live/Dead Staining Kit to perform Live / Dead assay bacteria - Escherichia coli

Get tips on using Bacteria Live/Dead Staining Kit to perform Live / Dead assay bacteria - Corynebacterium glutamicum

Get tips on using Bacteria Live/Dead Staining Kit to perform Live / Dead assay bacteria - Bacillus subtilis