siRNA / miRNA gene silencing Human SPC-A1

Get tips on using ScaI R6211 to perform Restriction Enzymes ScaI

Get tips on using FastDigest ScaI to perform Restriction Enzymes ScaI

Get tips on using FastDigest SchI to perform Restriction Enzymes SchI / MlyI

Get tips on using ScaI restriction enzyme to perform Restriction Enzymes ScaI

Get tips on using Rat SCF ELISA to perform ELISA Rat - SC

Get tips on using Rat SCF PicoKine™ ELISA Kit to perform ELISA Rat - SC

Get tips on using Mouse Sca-1/Ly6 APC-conjugated Antibody to perform Flow cytometry Anti-bodies Mouse - Ly-6A-E/Sca1

There are a plethora of detection methods of cell cytotoxicity and proliferation by flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Pacific Blue™ anti-mouse Ly-6A/E (Sca-1) Antibody to perform Flow cytometry Anti-bodies Mouse - Ly-6A-E/Sca1

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.