Immunofluorscence  53BP1 [H-300]

Get tips on using Anti-trimethyl-Histone H3 (Lys4) Antibody to perform ChIP H3K4Me3 - Sheep Rat -NA-

Get tips on using Anti-phospho-Histone H2A.X (Ser139) Antibody to perform Immunohistochemistry γH2AX - Mouse Human -NA-

Get tips on using APC-H7 Mouse Anti-Human CD43 to perform Flow cytometry Anti-bodies Human - CD43

Get tips on using APC-H7 Mouse Anti-Human CD71 to perform Flow cytometry Anti-bodies Human - CD71

Get tips on using APC-H7 Mouse Anti-Human CD44 to perform Flow cytometry Anti-bodies Human - CD44

Get tips on using Human Genome CGH Microarray Kit, 4x44K to perform Microarray Comperative genomic hybridization - Human HeLa

Get tips on using Human Genome CGH Microarray Kit 244A to perform Microarray Comperative genomic hybridization - Human HepG2

Get tips on using Kapa Biosystems HIFI HOTSTART READY MIX to perform PCR Hot start PCR - Bacterial DNA

Get tips on using Anti-trimethyl-Histone H3 (Lys27) Antibody to perform ChIP H3K27me3 - Sheep Rat YFP Tag

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.