RNA sequencing Human

Get tips on using MagMAX™-96 Total RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Duodenum

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - human peripheral blood mononuclear cells (PBMCs)

Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - human colorectal adenocarcinoma cells (CL-187)

Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Cells - primary human endometrial stromal cells

Get tips on using Quant-iT™ RiboGreen™ RNA Assay Kit to perform RNA quantification Fuorimetric - human blood

Get tips on using RediPlate™ 96 RiboGreen™ RNA Quantitation Kit to perform RNA quantification Fuorimetric - human DCs

Get tips on using Quant-iT™ RiboGreen™ RNA Assay Kit to perform RNA quantification Fuorimetric - human CSF

Get tips on using Quant-iT™ RiboGreen™ RNA Assay Kit to perform RNA quantification Fuorimetric - human plasma