Get tips on using Atg12 (D88H11) Rabbit mAb to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)
Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - K562 cells
Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - THP 1
Get tips on using SQSTM1/p62 (D5E2) Rabbit mAb to perform Autophagy assay cell type - THP 1
Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - SH-SY5Y
Get tips on using MasterPureTM-Yeast DNA Purification kit to perform DNA isolation / purification Yeast - Candida albicans
Get tips on using MasterPureTM-Yeast DNA Purification kit to perform DNA isolation / purification Yeast - Cryptococcus neoformans
Get tips on using MasterPureTM-Yeast DNA Purification kit to perform DNA isolation / purification Yeast - Pichia pastoris
Get tips on using MasterPureTM-Yeast DNA Purification kit to perform DNA isolation / purification Yeast - Saccharomyces cerevisiae
A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
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