Protein expression and purification Mammalian cells HEK 293

Get tips on using High Pure PCR Template Preparation Kit to perform DNA isolation / purification Tissue - placenta

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

Get tips on using miRCURY Exosome Cell/Urine/CSF Kit to perform Purification of extracellular vesicles Exosomes - Seminal plasma

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using AllPrep DNA/RNA/miRNA Universal Kit to perform RNA isolation / purification Tissue - mouse adipose tissue

Get tips on using AllPrep DNA/RNA/miRNA Universal Kit to perform RNA isolation / purification Tissue - human adipose tissue

Get tips on using Direct-zol RNA Kits to perform RNA isolation / purification Bacteria - Gram positive Streptococcus pyogenes

Get tips on using TRIzol™ LS Reagent to perform RNA isolation / purification Bacteria - Gram positive Streptococcus pyogenes

Get tips on using TRIzol™ LS Reagent to perform RNA isolation / purification Bacteria - Gram positive Listeria monocytogens

Get tips on using TRI Reagent™ Solution to perform RNA isolation / purification Bacteria - Gram negative Salmonella typhi