rna-isolation-purification-cells-immortalized-cos-7

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using MEBMTM Mammary Epithelial Cell Growth Basal Medium to perform 3D Cell Culture Media Mouse primary breast ephitelial cells-Mammospheres

Get tips on using MEBMTM Mammary Epithelial Cell Growth Basal Medium to perform 3D Cell Culture Media Human primary breast ephitelial cells-Mammospheres

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Get tips on using In Situ Cell Death Detection Kit, TMR red to perform TUNEL assay cell type - HEK293 human embryonic kidney cells

Get tips on using LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit to perform Live / Dead assay mammalian cells - mouse, T-cell

Get tips on using Senescence β-Galactosidase Staining Kit - Cell Signaling to perform Reporter gene assay β-galactosidase substrates - mouse pancreatic stellate cells

Get tips on using Senescence β-Galactosidase Staining Kit - Cell Signaling to perform Reporter gene assay β-galactosidase substrates - mouse mesenchymal stem cells

Get tips on using STEMdiff™ Hematopoietic Kit to perform Stem cell Differentiation media hiPSCs differentiation into CD43+ primitive hematopoietic progenitor cells (HPCs)

Get tips on using MammoCult™ Human Medium Kit to perform 3D Cell Culture Media Human breast cancer MDA-MB-231 cells-Mammospheres