shRNA gene silencing Mouse Prostate cancer cell lines (DU145 and PC3)

Get tips on using Senescence Detection Kit I (histochemical) to perform Reporter gene assay β-galactosidase substrates - HUVEC

Get tips on using Anti-Human CD56 (NCAM) APC-eFluor® 780 to perform Flowcytometry CD56 (NCAM) - Mouse / IgG1, kappa Human APC-eFluor 780

Get tips on using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® to perform RNA sequencing Mouse - ESCs (Embryonic Stem Cells)

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using QuikChange Site-Directed Mutagenesis Kit, 10 Rxn to perform Site Directed Mutagenesis (SDM) Mouse - 3T3-L1 Clk1

Get tips on using FlashTag™ Biotin HSR RNA Labeling Kits to perform Microarray RNA amplification & Labeling - Mouse skin tissue Biotin

Get tips on using FlashTag™ Biotin HSR RNA Labeling Kits to perform Microarray RNA amplification & Labeling - Mouse mammary tissue Biotin

Get tips on using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® to perform RNA sequencing Mouse - C2C12

Get tips on using Luminescent β-galactosidase Detection Kit II to perform Reporter gene assay β-galactosidase substrates - HEK293 human embryonic kidney cells