Site Directed Mutagenesis (SDM) Human Deletion PC-3

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Get tips on using Silencer® Select Negative Control No 1 siRNA to perform siRNA / miRNA gene silencing Mouse - siRNA negative control polymer / lipid

Products Thermo Fisher Scientific Silencer® Select Negative Control No 1 siRNA

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Immortalized cell lines 3T3-L1

Get tips on using Comet Assay to perform DNA Damage Assay A-375

Products Sigma-Aldrich Comet Assay

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - 3T3-L1

Products Sigma-Aldrich RIPA Buffer

Get tips on using Advanced DMEM to perform 3D Cell Culture Media Murina esophageal organoids

Products Thermo Fisher Scientific Advanced DMEM

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized NIH-3T3

Products Thermo Fisher Scientific TRIzol Reagent

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized C6/36

Products Thermo Fisher Scientific TRIzol Reagent

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Mouse - 3T3-L1

Products Illumina TruSeq Stranded mRNA

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Mouse - NSC-34

Products Illumina TruSeq Stranded mRNA

Get tips on using Beclin-1 Antibody to perform Autophagy assay cell type - BRL-3A

Products Cell Signaling Technology Beclin-1 Antibody

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