Immunohistochemistry Wilms Tumor 1 (WT1)

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Get tips on using VEGF A - AB-90040 to perform Immunohistochemistry Mouse - VEGFA

Products Immunological Sciences VEGF A - AB-90040

Get tips on using Anti-Insulin antibody (ab7842) to perform Immunohistochemistry Mouse - Insulin

Products Abcam Anti-Insulin antibody (ab7842)

Get tips on using Anti-Insulin antibody (ab7842) to perform Immunohistochemistry Mouse - Insulin

Products Abcam Anti-Insulin antibody (ab7842)

Get tips on using Insulin (Autostainer Link 48) to perform Immunohistochemistry Mouse - Insulin

Products Agilent Technologies Insulin (Autostainer Link 48)

Get tips on using NOTCH1/activated Notch1 Antibody to perform Immunohistochemistry Mouse - Notch1

Products Bioss NOTCH1/activated Notch1 Antibody

Get tips on using Lysozyme EC 3.2.1.17 (Concentrate) to perform Immunohistochemistry Mouse - Lysozyme

Products Agilent Technologies Lysozyme EC 3.2.1.17 (Concentrate)

RNA siRNA / miRNA gene silencing Human HeLa EPAS-1 Lipid

RNA siRNA / miRNA gene silencing Rat Glial cells GLT-1

RNA siRNA / miRNA gene silencing Rat Neuronal cells MCP-1

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Live / Dead assay mammalian cells INS-1 832/12

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