RNA isolation / purification Tissue Human

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Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Mouse Muscle

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Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Mouse Liver

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Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Tissue - Mouse Larynx

Products Thermo Fisher Scientific PicoPure™ RNA Isolation Kit

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Tissue - Mouse Kidney

Products Roche Lifesciences High Pure RNA Isolation Kit

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Mouse Heart

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Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Mouse Adipose

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Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA Microarray RNA amplification & Labeling Human brain tissue Cyanine 3

RNA RNA isolation / purification Water samples

RNA RNA isolation / purification Plants Seeds

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary bovine aortic endothelial cells

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