Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Mouse Muscle
Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Mouse Liver
Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Tissue - Mouse Larynx
Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Tissue - Mouse Kidney
Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Mouse Heart
Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Mouse Adipose
Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.
When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
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