siRNA / miRNA gene silencing Mouse B16 Melanoma cells

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Get tips on using Gibco™StemPro™ Chondrogenesis Differentiation Kit to perform Stem cell Differentiation media hBMSCs differentiation into chondrogenic cells

Products Thermo Fisher Scientific Gibco™StemPro™ Chondrogenesis Differentiation Kit

Get tips on using X-tremeGENE™ HP DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines PANC-1

Products Sigma-Aldrich X-tremeGENE™ HP DNA Transfection Reagent

Get tips on using ApopTag Fluorescein in Situ Apoptosis Detection Kit to perform TUNEL assay cell type - HEK293 human embryonic kidney cells

Products Millipore ApopTag Fluorescein in Situ Apoptosis Detection Kit

Get tips on using TdT In Situ Apoptosis Detection Kit - Fluorescein to perform TUNEL assay cell type - HeLa cells human cervical cancer

Products R&D system, Minneapolis, MN, USA TdT In Situ Apoptosis Detection Kit - Fluorescein

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - K562 human leukemia cells

Products Enzo Life Sciences ROS-ID® Total ROS/Superoxide detection kit

Get tips on using OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) to perform ROS assay cell type - K562 human leukemia cells

Products Cell Biolabs OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence)

Get tips on using MasterPure™ Complete DNA and RNA Purification Kit to perform DNA isolation / purification Cells - Immortalized cell lines C2C12

Products Epicentre MasterPure™ Complete DNA and RNA Purification Kit

Get tips on using PE Annexin V Apoptosis Detection Kit with 7-AAD to perform Apoptosis assay cell type - Human T-cells

Products BioLegend PE Annexin V Apoptosis Detection Kit with 7-AAD

Get tips on using CellROX™ Deep Red Reagent, for oxidative stress detection to perform ROS assay cell type - human umbelical vein endothelial cells (HUVEC)

Products Thermo Fisher Scientific CellROX™ Deep Red Reagent, for oxidative stress detection

DNA damage assay is a standard method for determining in-vivo/in-vitro genotoxicity by measuring the breaks in the DNA chain of animal and plant cells. Initial DNA damage leads to cell cycle arrest and, at the final stages, leads to induction of senescence or cell death (apoptosis, necrosis, autophagy, or mitotic catastrophe). Detection of DNA damage from mild to moderate to severe is challenging when studying genotoxicity in the pool of cells. It is favorable to use DNA damage assay kits available for prominent identification of the extent of damage in the analysis.

Cellular assays DNA Damage Assay Human Skin Fibroblast Cell (FSK)

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