siRNA / RNAi /miRNA transfection Human Cells HeLa

Get tips on using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 to perform ChIP Mouse - 3T3-L1 cells

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® to perform RNA sequencing Mouse - ESCs (Embryonic Stem Cells)

Get tips on using Mesenchymal Stem Cell Chondrogenic Differentiation Medium to perform Stem cell Differentiation media hBMSCs differentiation into chondrogenic cells

Get tips on using Mesenchymal Stem Cell Chondrogenic Differentiation Medium to perform Stem cell Differentiation media hUMSCs differentiation into chondrogenic cells

Get tips on using Mesenchymal Stem Cell Adipogenic Differentiation Medium to perform Stem cell Differentiation media hUMSCs differentiation into adipogenic cells

Get tips on using MEGMTM Mammary Epithelial Cell Growth Medium BulletKitTM to perform 3D Cell Culture Media BT-549 cells-Mammospheres

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - Mouse skeletal muscle cells

Get tips on using Cell Meter™ Autophagy Assay Kit *Green Fluorescence to perform Autophagy assay cell type - Mesenchymal stromal cells

Get tips on using LIVE/DEAD Fixable Violet Dead Cell Stain Kit to perform Live / Dead assay mammalian cells - HEK 293