siRNA / miRNA gene silencing Human hES cell line H1 (WA01)

Get tips on using Difco™ Brain Heart Infusion Agar, BD to perform Bacterial cell culture media Escherichia coli

Get tips on using Difco™ Brain Heart Infusion Agar, BD to perform Bacterial cell culture media Streptococcus pyogenes

Get tips on using Difco™ Brain Heart Infusion Agar, BD to perform Bacterial cell culture media Enterococcus faecalis

Get tips on using BBL™ Brain Heart Infusion Broth, BD to perform Bacterial cell culture media Clostridum botulinum

Get tips on using Difco™ Brain Heart Infusion Agar, BD to perform Bacterial cell culture media Clostridium difficile

Get tips on using Difco™ Brain Heart Infusion Agar, BD to perform Bacterial cell culture media Bacillus cereus

Get tips on using Difco™ Brain Heart Infusion Agar, BD to perform Bacterial cell culture media Bacillus anthracis

Get tips on using RPMI 1640, with L-Glutamine and 25mM HEPES to perform Mammalian cell culture media DU145

Get tips on using RPMI 1640, with L-Glutamine and 25mM HEPES to perform Mammalian cell culture media VCaP

Generally isolating RNA from Gram-negative bacteria is easy, however keeping your working environment clean and RNase free (use RNase inhibitor) is essential. Some common points to keep in mind: a) Use fresh samples for isolation or store them by freezing in RNA stabilizing buffer until use. b) Choose the bacterial input amounts carefully, to ensure buffer volumes are adequate and not to overload the columns.